Cfr gene linezolid resistance
Linezolid is an effective antimicrobial agent to treat methicillin-resistant Staphylococcus aureus (MRSA). Resistance to linezolid due to the cfr gene is described worldwide. The present study aimed to analyze the prevalence of the cfr–mediated linezolid resistance among MRSA clinical isolates in our area. A very low prevalence of cfr mediated linezolid resistance was found: only one cfr gene was the main mechanism of linezolid resistance in staphylococci isolates.. cfr gene resided on plasmids similar to cfr-carrying plasmids detected in animal pathogens.. cfr gene was flanked by mobile elements, indicating horizontal gene transfer.. Incidence of linezolid-resistant coagulase-negative staphylococci is of great concern.The cfr-like cfr(C) gene was cloned in C. difficile 630Δerm in which it conferred PhLOPS A resistance. In C. bolteae 90B3: (i) qRT-PCR analysis indicated that cfr( C) was similarly expressed in the absence or presence of either chloramphenicol or clindamycin or linezolid; and (ii) cfr( C) was part of a putative 24 kb-transposon, which generated a detectable circular intermediate.The cfr (chloramphenicol-florfenicol resistance) gene encodes a 23S rRNA methyltransferase that confers resistance to linezolid. Detection of linezolid resistance was evaluated in the first cfr Acquired linezolid resistance genes, described so far, include cfr, cfr(B), cfr(C), optrA and poxtA. The cfr gene, first identified on the pSCFS1 plasmid from Staphylococcus sciuri ( Schwarz et al., 2000 ) encodes the Cfr methylase, which modifies the C-8 position of A2503 residue in 23S rRNA ( …G2576T mutations in the 23S rRNA gene were detected only in E. faecium [14 isolates; 71.4 % (10/14) linezolid-resistant and 28.6 % (4/14) linezolid-intermediate]. One linezolid-intermediate E. faecium harboured a L22 protein alteration (Ser77Thr). No isolates contained cfr or cfr(B) genes and any L3 or L4 protein alterations. No genetic mechanism of resistance was identified for 67.6 % (23/34 01/10/2004Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis, Resistance to Linezolid Is Mediated by the cfr Gene in the First Report of an Outbreak of Linezolid-Resistant Staphylococcus aureus. Omadacycline for Acute Bacterial Skin and Skin Structure Infections. Multiparameter Responses to Tedizolid Monotherapy and Moxifloxacin Combination Therapy viagra Models …Linezolid resistance occurs by mutations in the linezolid 23S rRNA binding site, the ribosomal proteins L3 and/or L4 of the peptide translocation centre of the ribosome or by acquisition of a plasmid-borne ribosomal methyltransferase gene, cfr. 22,23 All three mechanisms have been documented in LRSA and LRCoNS. 24 Sixty-three LRSA (Figure 2) and 322 LRCoNS (Figure 2) were investigated for Linezolid resistance, as described in numerous earlier reports, has been mediated by mutations in 23S rRNA or other ribosomal protein genes, implying the slow dissemination of resistance by these mechanisms ().However, the detection of a plasmid-borne cfr-mediated linezolid resistance gene in staphylococci recovered from human specimens in the United States adds a new dimension to the …
